Specialized Workshop Th2Thursday September 29, 2022: 7am - 5:30pm - Lecture List

"Flow Cytometry Bioanalysis - Rising of the 3rd Most Common/Important Technology in Bioanalytical Labs"

Importance of learning Flow, Mass, Imagine & Spectral Cytometry Bioanalysis and Major Applications in Biomarkers, Immunogenicity, Cell Therapy, Vaccine, Omics and Non-Liquid Matrices; Novel Techniques, Autogating Developments and Innovation in High Dimensional Cytometry - Social Networking Reception and Dinner is also Included - Flow Cytometry Day and Night!

Part 1: Understanding the Cytometry Conventional/Novel Technologies and Main Applications

  • Lecture 1:

    Importance of Conventional Flow Cytometry Bioanalysis for Clinical Trials – Past, Present, Future: Technology Introduction & Main Applications in Immunophenotyping, RO and PD Assays

  • Lecture 2:

    Importance of Spectral Cytometry for Biomarker Development – Current Status and Future Developments: Technology Introduction & Primary Unique Features vs Conventional Flow Cytometry, Main Application in Drug Discovery & Development

  • Lecture 3:

    Importance of Mass Cytometry for Bioanalytical Needs – Past, Present, Future: Technology Introduction, Primary Unique Features vs Conventional Flow Cytometry & Applications in Bioanalysis & Biomeasures

  • Lecture 4:

    Importance of Imaging Cytometry for Biomeasures – Past, Present, Future: Technology Introduction & Applications in Cell-based Biomeasure Assays

Part 2: Current Challenges with Validation & Sample Collection

  • Lecture 5:

    Strategies for Rationalizing Fit-for-purpose Criteria for Flow Cytometry Biomarker Assay Validation (BAV): How to Determine what Parameters/Acceptance Criteria are really needed without overdoing or underdoing?

  • Lecture 6:

    Turning the CLSI H62 into Practise for Bioanalytical/Clinical Laboratories: First-hand experience in trying to apply the CLSI H62 to real situations with pros & cons

  • Lecture 7:

    Challenges and Strategies for Clinical Sample Collection in Flow Cytometry and Cell-Based Assays

Part 3: Specific Complex Applications

  • Lecture 8:

    Flow Cytometry in the Monitoring of Immune Responses to Vaccines: Method Development challenges and Assay Optimization Strategies to Increases the Robustness and Accuracy of the Data

  • Lecture 9:

    Use of Model Antigens to Validate Antigen Specific Vaccine Flow Cytometry Assays

  • Lecture 10:

    Challenges and Value of Clinical Receptor Occupancy (RO) Assessments for Bispecific T Cell redirection by Flow Cytometry

  • Lecture 11:

    Challenges in the Development of Novel Flow Cytometry Assays for Bioanalysis of PD Markers: RO of Small Molecule inhibitor, Tumor Tissue and Phagocytosis Measurements

Part 4: Innovation in Cytometry

  • Lecture 12:

    Can we use Cytometry in Tissues Bioanalysis? Current Challenges, Innovative Approaches and Technologies

  • Lecture 13:

    Recent Advancements in Fit-for-purpose Validation Requirements for High Parameter Cytometry Assays with/without Autogating

  • Lecture 14:

    Current Evolution of High Dimensional Flow Cytometry Data in Clinical Settings and needs for Autogating

Part 5: White Paper in Bioanalysis

  • 2022 White Paper on Cytometry Topics:

    Consensus & Conclusions on Cytometry Topics for 2022 White Paper

Agenda at a Glance Agenda at a Glance